Flowjo Student License
The DUKE FlowJo Site License is solely for the use of DUKE faculty, students, and staff
- The license agreement requires each FlowJo user to register their computer's Hardware Address with the site license manager (DHVI Flow Facility). See instructions below on how to obtain your computer's Hardware Address. Note: you will be able to run FlowJo ONLY on the computer specified by the Hardware Address.
- Use the link below to add a new FlowJo license, to move an existing license from one computer to another, to change the user name or fund code associated with a FlowJo license, or to cancel a FlowJo license.
- Please be sure that you are linked to a project (fundcode or PO#) in CoreResearch@Duke.
How to find your Hardware Address:
- PC: Start => Run => CMD => ipconfig/all => Local Area => Physical Address
- Mac: Apple => About this Mac => More info => Network => Locations => Ethernet 1: => Hardware (MAC) Address
Flowjo Student License Verification
To request or modify a FlowJo Site License, click here.
Flowjo Free Student License Average ratng: 3,0/5 2657 votes. Free Trial Download it. You will then receive a license code which you can put into the software. After submitting this web form, you will receive an email containing a FlowJo® Serial Number (good for 30 days) which you can paste into FlowJo®. Getting Started and FlowJo Licensing. Background and Objectives: Alpha-1 antitrypsin is a serine protease inhibitor that demonstrates an array of immunomodulatory functions. Individuals with the genetic condition of alpha-1 antitrypsin deficiency (AATD) are at increased risk of early onset emphysematous lung disease. This lung disease is partly driven by neutrophil mediated lung destruction in an environment of low AAT. The license is not a hardware dongle but rather a license linked to your computer. We recommend these licenses be linked to shared laboratory computers. This site license allows access to all available versions of FlowJo ™. To activate the FlowJo ™ analysis software site license, take the following steps: Designate the computer for.
FlowJo Help (Treestar Inc.)
On-Campus Facilities and Experts
Ithaca
Flow Cytometry instrumentation is available in a main facility in 140 Biotechnology Building and a satellite facility in VRT T2-003 of the College of Veterinary Medicine. The facility houses four cell sorters and three analyzers. Read more at the CIB Flow Cytometry Core Facility Website
Contact: Lydia Tesfa
Weill Cornell
The WCM CLC Flow Cytometry Core Facility offers state-of-the-art fluorescence activated cell sorting (FACS) instruments and services, and expertise in their applications, to the Weill Cornell Medicine (WCM) community and to outside investigators. The facility also provides consultation on project design and data analysis, and offers educational workshops, seminars and training. Read more at the WCM CLC Flow Cytometry Core Facility Website
Contact: Jason McCormick or Tom Baumgartner – 646-962-7611
Panel Design
Ithaca Specific
• Fluorofinder Panel Design
• Panel Design Dos & Don’ts – L. Tesfa
Other
• Astrolabe Antibody Staining Data Set
• BD Guided Panel Solution
• BD Spectrum Viewer
• BenchSci Ab Search
• Bio-Rad Antigen Density
• Chromocyte Panel Builder
• Fluorofinder
• Thermo Spectra Viewer
• Thermo Panel Builder
• UC Flow Panel Design Overview
Data
Analysis
The Flow Cytometry Core Facility has partnered with Weill Cornell Medicine and the BRC Bioinformatics facility for access to FlowJo and FCS Express software. If you are interested in acquiring a license for FlowJo or FCS Express, please contact bio-it@cornell.edu. Please include your name, your PI’s name and a valid Cornell account number. (http://www.biotech.cornell.edu/node/137). FlowJo software is also available on the Flow Cytometry Facility Shared workstation for any user of the facility subject to schedule availability and access to the lab.
Repositories
• FlowRepository
• ImmPort
• ImmuneSpace
• Minimum Set of Research Flow Cytometry Standards (MiSET RFC)
• Minimum Information about a Flow Cytometry Experiment (MIFlowCyt)
Textbooks
• Practical Flow Cytometry – Howard Shapiro
📘 Physical copy | Digital copy
• Flow Cytometry: First Principles – Longobardi
Digital copy
• Imaging Flow Cytometry: Methods and Protocols – Barteneva & Vorobjev
Digital copy
Flowjo Student License Application
• Flow Cytometry: Principles and Applications – Macey
📘 Physical copy | Digital copy
Virtual Resources
Communities
• Chromocyte
• International Society for Advancement of Cytometry
• Scientific Community Imaging Forum
• Purdue University Cytometry Laboratories
Tips, Tutorials, and Protocols
• BD Biosciences – Flow Cytometry Web-Based Training
• BioLegend for Spectral Cytometry
• Bio-Rad – Introduction to Flow Cytometry
• Boster Bio – Flow Technical Resource Center
• CCEN e-learning
• Compensation in Flow Cytometry Analyses – Roederer
• Current Protocols in Cytometery and Immunology – Wiley
• Cytek Full Spectrum Viewer
• CYTO U – International Society for Advancement of Cytometry
• Expert Cytometry
• Flowbook – FCS Express
• FlowJo University
• FlowJo Webinars
• Protocols for Fluorescence Conjugation of Antibodies – Roederer
In-person Training Opportunities
Flowjo Free Student License
• Annual Course in Flow Cytometry
Offered by the University of Wisconsin, Madison Campus
• Comprehensive Course in Flow Cytometry
Offered by the University of California, Davis Campus
• Annual Practical Course in Clinical Cytometry
Offered by the Clinical Cytometry Education Network
Vendor Websites
• BDbiosciences
• BioLegend
• ThermoFisher
• Miltenyi Biotec
• Novus Biologicals
• Sigma-Aldrich
• Beckman Coulter